Figure 3.

Generation of CAR-PSCA T cells. (A) Schematic of the protocol used to activate and transduce CAR-PSCA T cells in vitro. PBMCs, peripheral blood mononuclear cells. (B) Retroviral vector map of the CAR-PSCA construct. (C) Transduction efficiency of CAR-PSCA transduced T cells from a representative donor as evaluated by flow cytometry using an antibody directed against the CH2CH3 region of the retroviral construct. (D) T cells generated from both normal donors (n = 7) and pancreatic cancer patients (n = 3) were efficiently transduced