Figure 4.
Chimeric antigen receptor (CAR) prostate stem cell antigen (PSCA) T cells are cytotoxic in vitro. (A) To determine whether the CAR-PSCA T cells could lyse tumour antigen-expressing target cells, we tested the ability of a line generated from a representative donor to kill PSCA-expressing tumour cell lines PL45, CAPAN-1 and CFPAC in a standard 6-h Cr51 release assay. The PSCA negative cell line 293T served as a control. Effector : target (E : T) ratios of 40 : 1, 20 : 1, 10 : 1 and 5 : 1 were tested. (B) Summary of results using CAR-PSCA T cells from four healthy donors. (C) Summary of results using CAR-PSCA T cells from four pancreatic cancer patients. 
, CAR-PSCA cells; 
, non-treated T cells