Table 2.
Gene | Sex | Model | AICb | GOFc | BMDd | BMDLd |
---|---|---|---|---|---|---|
Kim-1/Havcr1 | Male | Hill | 456.8 | >0.999 | 13.5 | 9.2 |
Linear | 472.2 | 0.0007 | 4.3 | 3.5 | ||
Polynomial | 467.4 | 0.006 | 7.2 | 4.8 | ||
Power | 465.9 | 0.011 | 7.6 | 5.4 | ||
Female | Hill | 201.2 | 0.997 | 16.2 | 9.7 | |
Linear | 221.9 | <0.0001 | 5.0 | 4.0 | ||
Polynomial | 201.0 | 0.601 | 11.6 | 8.3 | ||
Power | 201.4 | 0.527 | 10.8 | 8.4 | ||
Timp1 | Male | Hill | 66.9 | 0.967 | 10.2 | 8.7 |
Linear | 99.3 | <0.0001 | 6.6 | 5.2 | ||
Polynomial | 99.4 | <0.0001 | 4.5 | 3.1 | ||
Power | 99.3 | <0.0001 | 6.6 | 5.2 | ||
Female | Hill | 50.0 | 0.984 | 15.5 | 9.4 | |
Linear | 58.5 | 0.031 | 6.9 | 5.5 | ||
Polynomial | 60.2 | 0.015 | 8.0 | 4.8 | ||
Power | 59.6 | 0.021 | 9.0 | 5.7 | ||
Clu | Male | Hill | 207.3 | 0.006 | 10.3 | 8.7 |
Linear | 249.7 | <0.0001 | 7.9 | 6.2 | ||
Polynomial | 246.1 | <0.0001 | 3.9 | 2.7 | ||
Power | 249.7 | <0.0001 | 7.9 | 6.2 | ||
Female | Hill | 189.4 | 0.481 | 10.7 | 8.8 | |
Linear | 208.5 | <0.0001 | 9.5 | 7.2 | ||
Polynomial | 207.7 | 0.0001 | 5.3 | 3.5 | ||
Power | 208.5 | <0.0001 | 9.5 | 7.2 | ||
Lcn2 | Male | Hill | 139.7 | >0.999 | 15.9 | 9.6 |
Linear | 178.7 | <0.0001 | 4.4 | 3.5 | ||
Polynomial | 143.0 | 0.354 | 10.5 | 7.9 | ||
Power | 143.8 | 0.248 | 9.5 | 7.7 | ||
Female | Hill | 120.8 | 0.970 | 15.2 | 9.8 | |
Linear | 137.7 | 0.0003 | 5.4 | 4.4 | ||
Polynomial | 119.9 | 0.777 | 12.6 | 8.9 | ||
Power | 120.2 | 0.691 | 11.7 | 8.9 | ||
Spp1 | Male | Hill | 148.6 | 0.987 | 12.1 | 8.9 |
Linear | 175.9 | <0.0001 | 5.6 | 4.5 | ||
Polynomial | 177.4 | <0.0001 | 4.7 | 3.2 | ||
Power | 177.8 | <0.0001 | 6.0 | 4.5 | ||
Female | Hill | 44.2 | 0.506 | 10.6 | 8.8 | |
Linear | 69.3 | <0.0001 | 8.3 | 6.4 | ||
Polynomial | 69.1 | <0.0001 | 5.1 | 3.4 | ||
Power | 69.3 | <0.0001 | 8.3 | 6.4 |
Benchmark doses (BMDs) and the lower 95% confidence limits (BMDLs) were calculated using Environmental Protection Agency Benchmark Dose Software (version 2.1.1; http://www.epa.gov/ncea/bmds). The calculations were conducted using Hill, linear, polynomial, and power models to fit the relative gene expression levels of Kim-1/Havcr1, Timp1, Clu, Lcn2, and Spp1 (mean ± SD; n = 6) and the mean doses of melamine and cyanuric acid from the entire seven-day study. The BMD was defined as the dose corresponding to a change in the mean response equal to one control standard deviation from the control mean.
AIC, Akaike information criterion.
GOF, Goodness of fit.
Relative gene expression.