Figure 3.

Individual molecules were modeled as wormlike chains with a persistence length, A, of 14 nm and a contour length of 120 nm. If the stochastic model resulted in unfolding of one of the FnIII modules constituting the molecule (red sphere), a segment with a contour length of 32 nm and persistence length of 0.42 nm was added in series to the original molecule. The contour length of the original molecule was reduced by 3.5 nm.