Table 1.

Values used to describe the probability of FnIII module unfolding are listed for those modules that were included in the in silico fiber-stretching experiment

Module	k0u (s−1)	Δxu (nm)	Ref.
III1	0.004	0.17	(16)
III2	0.004	0.17	(16)
III10	0.02	0.38	(16)
III13	0.022	0.34	(16)
III12	0.022	0.28	(16)
III7	0.022	0.19	(31)
III8	0.02	0.32	(31)
III9	0.02	0.3	(31)
III14	0.02	0.375	(31)
T-FnIII3	0.022	0.29	(30)

Note that k⁰_u and Δx_u were given explicitly by Oberhauser et al. (16) for III1, III2, III10, and III13. The remaining values were estimated to match published data on the most likely unfolding force with the mechanical hierarchy in Craig et al. (31).