Table 1.
Percentage of Ae. aegypti showing infection, dissemination (legs and wings from infected bodies), and transmission of MAYV after exposure to an artificial blood meal at various dilutions or viremic adult mice
| Blood meal titers (PFU/mL) | Percent infected of total (bodies) | Percent disseminated of infected (legs and wings) | Percent transmitting of infected (oil droplet or blood sample from mouse)* | ||
|---|---|---|---|---|---|
| Artificial feeder | Viremic mouse | Capillary tube | Suckling mouse | ||
| 7.34† | 84 (31/37) | 94 (29/31) | 88 (7/8) | 67 (2/3) | |
| 6.57‡ | 32 (10/31) | 80 (8/10) | 43 (3/7) | NT | |
| 6.28† | 41 (13/32) | 92 (12/13) | 100 (2/2) | NT | |
| 6.08† | 55 (16/29) | 94 (15/16) | 67 (2/3) | NT | |
| 5.85† | 44 (14/32) | 71 (10/14) | 100 (2/2) | NT | |
| 5.59‡ | 24 (7/29) | 71 (5/7) | 100 (3/3) | NT | |
| 5.57§ | 3 (1/32) | 100 (1/1) | 0 (0/2) | NT | |
| 5.45§ | 0 (0/29) | 0 | NT | NT | |
| 5.11† | 0 (0/22) | 0 | NT | NT | |
| 4.61§ | 0 (0/32) | 0 | NT | NT | |
| 4.36‡ | 0 (0/22) | 0 | NT | NT | |
| 4.30‡ | 0 (0/24) | 0 | NT | NT | |
| 4.26§ | 3 (1/32) | 100 (1/1) | NT | NT | |
| 3.73† | 0 (0/32) | 0 | NT | NT | |
| 3.36‡ | 0 (0/31) | 0 | NT | NT | |
| ND (< 2.00)‡ | 0 (0/15) | 0 | NT | NT | |
NT = not tested; ND = not detected.
*
Ten specimens from mosquitoes fed at each of seven infectious doses were sampled at 14 days post-feeding for a separate transmission study. Specimens were distinct from those specimens used to calculate the infection and dissemination rates.
†
One independent day of mosquito infection.
‡
A second independent day of mosquito infection.
§
A third independent day of mosquito infection.