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Energetic impact of Ala/Val mutations on the modulation of K-Shaw2 by halothane. (A) Apparent free-energy changes induced by single S4-S5 and S6 mutations (see legend to Fig. 3 for additional information). (B) Absolute coupling energies for double K-Shaw2 mutants (see legend to Fig. 3). The + sign above the tallest bar indicates that the coupling energy is estimated to be ≫5 kcal/mol. (C) Scatter plot of ΔΔG versus nH. The line is the best-fit linear regression (R2 = 0.87).
