Figure 2.

Fas TMD quantum-yield-weighted lifetimes as a function of X_lo in POPC/PSM/Chol membranes at room temperature with λ_exc = 295 nm and λ_em = 340 nm. The peptide concentration was 0.35 mol % (solid circles), 0.70 mol % (gray circles), and 3.0 mol % (open circles) of total lipid. The dashed lines serve as a guide to the eye. The error bars represent the standard deviations corresponding to at least three independent samples.(Inset) Nonlinear fit of Eq. 4 to the experimental data for 0.35 mol % peptide (from Fig. 2), with $K_{\text{p}}^{\text{lo}/\text{ld}}$ = 0.24 ± 0.02 (R² = 0.998), where $K_{\text{p}}^{\text{lo}/\text{ld}}$ is the Fas TMD partition coefficient between the lo and ld phases in POPC/PSM/Chol membranes at room temperature.