Figure 5.

Ceramide has minor effects on Fas TMD membrane organization. (A) Steady-state fluorescence anisotropy (〈r〉) of Fas TMD in POPC/PSM/Chol membranes containing 0 (solid circles), 2 (gray circles), and 4 (open circles) mol % PCer. (Inset) Fas peptide steady-state anisotropy values in membranes containing PCer calculated assuming no alterations in the fluorescence lifetime. (B) Quantum-yield-weighted lifetimes $\left(\overline{\tau }\right)$ of Fas TMD in the membranes in A. Errors bars represent standard deviations of at least three independent samples.