Table 1.

Time-resolved fluorescence anisotropy parameters of Fas TMD in ld and lo-rich vesicles

Xlo	〈r〉exp	ϕ1 (ns)	β1	ϕ2 (ns)	β2	r∞	χ2	〈r〉calc
0	0.090 ± 0.006	0.033	0.067	4.325	0.045	0.064	1.098	0.088
0.86	0.061 ± 0.005	—∗	—∗	0.861	0.021	0.055	1.145	0.061

Parameters include rotational correlation times, ϕ_i, amplitudes, β_i, and limiting anisotropy, r_∞. Vesicles with X_lo = 0 are ld and those with X_lo = 0.86 are lo-rich; measurements were made at room temperature. Also shown are the experimentally measured steady-state fluorescence anisotropy, 〈r〉_exp, and the steady-state anisotropy value calculated from the integration of the anisotropy decay, 〈r〉_calc. Peptide concentration was 0.35 mol % relative to total lipid.

^∗In lo-rich membranes, Fas TMD anisotropy decays were described by single-correlation rotational time (see text for further details).