Table 5. Inbreeding effect on the germination time of each seed (mixed effect ANCOVA model).
Num d.f. | Den d.f. | F | P-value | ||
---|---|---|---|---|---|
Fixed effects and covariates | |||||
Block | 3 | 527 | 0.89 | 0.4462 | NS |
Zone | 2 | 15 | 4.15 | 0.0368 | * |
Generation | 2 | 15 | 0.18 | 0.8407 | NS |
Probability of selfing (p.self) | 1 | 527 | 7.28 | 0.0072 | ** |
Generation × p.self | 2 | 527 | 6.31 | 0.0020 | ** |
Selfing effect within each generation | Estimate | 95% Confidence interval | |||
p.self within G0 | −1.010 | [−1.440; −0.581] | |||
p.self within G1 | 0.053 | [−0.392; +0.497] | NS | ||
p.self within G2 | −0.151 | [−0.672; +0.370] | NS | ||
Variance components | s.d. estimate | 95% Confidence interval | |||
Among progenies (at p.self=0) | 0.583 | [0.397; 0.856] | |||
Residual | 0.769 | [0.724; 0.817] |
The covariate p.self is the probability of each seed being selfed, which provides an estimate of inbreeding depression. The random variation among progenies includes variation of genetic values of the mother trees and maternal effects.
*P<0.05; **P<0.01.