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. 2010 Jul 28;106(4):614–624. doi: 10.1038/hdy.2010.94

Figure 1.

Figure 1

FISH mapping of S. cynthia orthologues of B. mori Z-linked genes on female pachytene chromosomes of three subspecies of S. cynthia. Red signals (arrowheads) are Cy3-labelled probes of BYB (a–e) and kettin (f–h) orthologous genes. GISH with Green-labelled female genomic probe (green signals) identified the original W compartment composed of heterochromatin (b–e, g, h) and also highlighted a block of heterochromatin in the NOR autosome (asterisk in b, d). Chromosomes were counterstained with DAPI (light blue). S. c. ricini (a, f): the U-shaped, apparently self-paired univalent of the Z chromosome with BYB orthologue signals on both chromatids is seen in the pachytene complement besides 13 autosome bivalents; also note a conspicuous nucleolus (N) associated with one end of the NOR autosome (a); and a Z-chromosome univalent with kettin orthologue signals on both chromatids (f). S. c. walkeri (b, c, g): a pachytene complement composed of a neo-Wneo-Z sex chromosome bivalent and 12 autosome bivalents (b); signals of the BYB (b, c) and kettin (g) orthologous probes are located on the neo-Z chromosome. S. cynthia subsp. indet. (d, e, h): a pachytene complement consists of a neo-sex chromosome trivalent (neo-WZ1Z2) and 11 autosome bivalents (d); signals of the BYB (d, e) and kettin (h) orthologous probes are located on the Z1 chromosome. Bar represents 10 μm (a–e, h), 7.5 μm (g) and 5 μm (f).