Table 3. Frequency of flax gene flow from 0.1–45 m distance from the pollen source at ElRS in 2006.
Mean distancea (m) | Seeds screenedb | Seeds with high ALA | Actual frequency of gene flow in sample | Ho frequency of gene flow p(x) | Power, (1−β); α=0.05%c |
---|---|---|---|---|---|
0.1 | 24 000 | 347 | 0.014458 | 0.005 | 0.95 |
0.5 | 24 000 | 201 | 0.008375 | 0.005 | 0.95 |
0.9 | 4800 | 4 | 0.000833 | 0.005 | <0.8 |
1.3 | 9600 | 21 | 0.002188 | 0.01 | 0.95 |
1.7 | 9600 | 47 | 0.004896 | 0.01 | 0.95 |
2.5 | 9600 | 6 | 0.000625 | 0.005d | <0.8 |
2.9 | 14 400 | 10 | 0.000694 | 0.005d | <0.8 |
4 | 48 000 | 60 | 0.00125 | 0.025 | 0.95 |
7 | 52 800 | 37 | 0.000701 | 0.001 | 0.85 |
15 | 148 800 | 22 | 0.000148 | 0.0005 | 0.95 |
25 | 168 000 | 17 | 0.000101 | 0.0005 | 0.95 |
35 | 100 800 | 11 | 0.000109 | 0.001 | 0.95 |
45 | 134 400 | 0 | 0.000000 | 0.0005 | 0.95 |
Abbreviations: ALA, α-linolenic acid; ElRS, Ellerslie Research Station.
Frequency of gene flow was calculated from the number of seeds screened and the number of high ALA seeds. To determine gene flow significance, the null hypothesis frequency, based on the sample size and power, was compared with the sample frequency.
Mean distance from the pollen source was used in analysis for all observations.
Total number of seeds screened from all (eight) blocks for a specific distance from the pollen source.
Value of power (1−β) was calculated for 95% confidence interval (α=5%) using equation (1) (see text) and Table 1.
α=5%.