Table 4. Frequency of flax gene flow from 0.1–45 m distance from the pollen source at EdRS in 2007.

Mean distancea (m)	Seeds screenedb	Seeds with high ALA	Actual frequency of gene flow in sample	Ho Frequency of gene flow p(x)	Powerc, (1−β); α=0.05%
0.1	9600	232	0.024167	0.01	0.95
0.5	9600	60	0.00625	0.01	0.95
1.3	9600	31	0.003229	0.01	0.95
2.5	14 400	26	0.001806	0.005	0.95
4	91 200	40	0.000439	0.001	0.95
7	115 200	44	0.000382	0.0005	0.95
15	96 000	29	0.000302	0.0005	0.85
25	460 600	13	0.000063	0.0001	0.85
35	176 800	0	0	0.00005	0.95
45	176 800	0	0	0.00005	0.95

Abbreviations: ALA, α-linolenic acid; EdRS, Edmonton Research Station.

Frequency of gene flow was calculated from the number of seeds screened and the number of high ALA seeds. To determine gene flow significance, the null hypothesis frequency, based on the sample size and power, was compared with the sample frequency.

^aMean distance from the pollen source was used in analysis for all observations.

^bTotal number of seeds screened from all (eight) blocks for a specific distance from the pollen source.

^cValue of power (1−β) was calculated for 95% confidence interval (α=5%) using equation (1) (see text) and Table 1.