Figure 2.

Electrical impedance of the RPE cell monolayer is decreased on expression of RapGAP. ARPE-19 cells expressing either GFP or RapGAP were plated at equal cell density into microelectrode-coated wells of an E-Plate 16 and electrical impedance was measured by RTCA. (A) Representative RTCA trace showing that Cell Index is lower in cells expressing RapGAP compared with GFP alone. Data points represent the average Cell Index ± SD from at least 3 separate wells. (B) Combined data from 3 independent experiments showing the average Cell Index ± SD at the 22-hour time point. RapGAP expression significantly decreases Cell Index at this time point (*P ≤ 0.01, n = 3 independent experiments).