Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Efficient RNAi-mediated knockdown of Rap1 expression in ARPE-19 using an engineered microRNA. ARPE-19 cells were infected with adenovirally-delivered, nontargeting control (Neg), or Rap1-knockdown virus. Three days later, cells were harvested and lysates were analyzed for Rap1 protein by Western blot analysis. Loss of Rap1 protein is evident in Rap1 knockdown lane compared with Neg control lane. Blot was reprobed with an antibody against β-actin as a control to confirm equal loading.
