Figure 6.

Knockdown of Rap1 GTPase decreases plateau TER levels. ARPE-19 cells were plated on Transwell filters for 24 hours before infecting with virus to induce expression of either negative control or Rap1 RNAi constructs. TER was measured before addition of virus (un), and then at times indicated for a total period of 5 days. Data are shown from one representative of three independent experiments. Cells with Rap1 knockdown have significantly lower TERs at all time points after knockdown. Bars represent average TER ± SD from n = 3 Transwells per condition (*P < 0.01).