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CEC transmigration is higher across Rap1 knockdown ARPE-19 cell monolayers. ARPE-19 cells preinfected with control or Rap1 knockdown virus were plated on inverted Transwells. Fluorescently-labeled CECs were added and allowed to transmigrate for 48 hours before quantification. Data are shown as number of transmigrated CECs × 104 cells/mL (mean of n = 5 Transwells ± SD). Graph shows one representative of three independent experiments (*P = 0.018862).
