Table 1.
Mouse Genotype | n | Age (wk) | Body Weight (g)* |
---|---|---|---|
RT-PCR, qRt-PCR, and Western Blot Analysis to Examine σR1 Gene/Protein Expression in Retina | |||
σR1+/+ (wild type) | 8 | 5–6 | 18.2 ± 0.3 |
σR1+/− (heterozygous) | 4 | 5–6 | 17.8 ± 0.2 |
σR1−/− (homozygous, knockout) | 8 | 5–6 | 17.2 ± 0.3 |
σR1+/+ (wild type) | 3 | 18 | 31.5 ± 1.0 |
σR1+/− (heterozygous) | 3 | 18 | 30.8 ± 1.0 |
σR1−/− (homozygous, knockout) | 3 | 18 | 31.3 ± 1.5 |
Immunohistochemistry to Examine σR1 Protein Expression in Retina and TUNEL Assay | |||
σR1+/+ (wild type) | 3 | 5–6 | 17.3 ± 2.0 |
σR1+/− (heterozygous) | 3 | 5–6 | 17.6 ± 0.6 |
σR1−/− (homozygous, knockout) | 3 | 5–6 | 17.4 ± 1.2 |
σR1+/+ (wild type) | 3 | 18 | 29.3 ± 1.6 |
σR1+/− (heterozygous) | 3 | 18 | 28.2 ± 1.6 |
σR1−/− (homozygous, knockout) | 3 | 18 | 28.6 ± 1.3 |
ERG Analysis of Retinal Function and Subsequent Light Microscopic/Morphometric Analysis (5 and 18 wk) | |||
σR1+/+ (wild type) | 7 | 5–6 | 18.3 ± 1.0 |
σR1+/− (heterozygous) | 8 | 5–6 | 17.4 ± 0.5 |
σR1−/− (homozygous, knockout) | 8 | 5–6 | 18.6 ± 0.8 |
σR1+/+ (wild type) | 6 | 18 | 28.9 ± 1.7 |
σR1+/− (heterozygous) | 6 | 18 | 29.8 ± 1.2 |
σR1−/− (homozygous, knockout) | 6 | 18 | 29.9 ± 0.9 |
Retinal Permeability Assays | |||
σR1+/+ (wild type) | 4 | 18 | 29.1 ± 1.9 |
σR1−/− (homozygous, knockout) | 2 | 18 | 26.2 ± 2.7 |
σR1−/− (homozygous, knockout) | 2 | 9 | 27.1 ± 1.1 |
Ultrastructural Analysis of Optic Nerve | |||
σR1+/+ (wild type) | 2 | 33 | 24.4 ± 1.9 |
σR1−/− (homozygous, knockout) | 2 | 33 | 27.8 ± 1.3 |
Assessment of Intraocular Pressure, ERG Analysis (59 wk), TUNEL Assay and Ultrastructure (59 wk) | |||
σR1+/+ (wild type) | 7 | 18–59 | 23.6 ± 0.5 to 37.0 ± 1.1 |
σR1+/− (heterozygous) | 3 | 18–59 | 27.9 ± 2.2 to 31.5 ± 5.3 |
σR1−/− (homozygous, knockout) | 8 | 18–59 | 26.4 ± 1.1 to 30.5 ± 1.3 |
Mean ± SEM.