Figure 3.
Transfection of fetal brain cells with siRNA specific to ERα significantly reduced ERα mRNA levels (A) but not ERβ levels (B). Transfection with siRNA specific to ERβ significantly reduced ERβ mRNA (B) but not ERα mRNA (A) as determined by qPCR by the ΔΔ CT method using β-actin as an internal control. Data is representative of triplicate samples from 3 independent experiments. ***, P<0.001 vs. control siRNA.