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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: FEMS Microbiol Lett. 2011 Jul 13;322(1):41–50. doi: 10.1111/j.1574-6968.2011.02330.x

Table 1.

Gene-specific primers used for PCR and RT-PCR analysis

TA system Primer sequence (5′ to 3′) Length (nt) Application
mazEFSa (+)ATCATCGGATAAGTACGTCAGTTT
(−)AGAAGGATATTCACAAATGGCTGA		 408 PCR, RT-PCR
parDEPa (+)GCGGCTGACCTGGATTTATC
(−)CCAAGCAGTAGCGGATCAATTG		 556 PCR, RT-PCR
relBEPa (+)CAGGGGGTAATTTCGACTCTG
(−)ATGAGCACCGTAGTCTCGTTC		 505 PCR
higBAPa (+)CTCATGTTCGATCTGCTTGC
(−)CAATGCTTCATGCGGCTAC		 469 PCR
relBEPa (+)CGCAGTACCTGGAAAGGCAGC
(−)GCCTTTAACCCGAAACGGG		 349 RT-PCR
higBAPa (+)GGCCAACATAGCATCAGGATC
(−)GGACGTATCAAAGTAACGCCC		 305 RT-PCR
mazEFSa Up (+)GTCTTGAACACATCTTCACGCG
(−)GCGAAAATACCGACACATGTAGAG		 753 PCR, flanking
mazEFSa Down (+)GCTTCGTTCGCTAGGGAGAG
(−)CTACAAGCGGGTGAGTCTGTAA		 556 PCR, flanking
parDPa Up (+)CGGTGATCTTTGCCAACACTAAG
(−)CTTCCGCTCAGCATATGACTC		 679 PCR, flanking
parEPa Down (+)TGAGTCTTCTGGGGGTGCTG
(−)GGAATTCCACACCATCCGC		 676 PCR, flanking
relEPa Up (+)CCGGAAAAAGCGCGAGAAAGC
(−)GGGGGCTGCAATGAGCCTG		 548 PCR, flanking
relBPa Down (+)GTGCTCATTTTCTGATCAACTTCG
(−)GTGACGCTCTCCGACAGCTTC		 646 PCR, flanking
higAPa Up (+)GATCCGACCCCTTCCGTCTAAACG
(−)GTAGCCGCATGAAGCATTG		 823 PCR, flanking
higBPa Down (+)CAGGTGGAGAGCGCAGGTC
(−)CAATTGTCCCAACGCCTCCTTCG		 712 PCR, flanking
higAPa Up-PA7 (+)GTTTGCCACGTTTGCATGCAG
(−)CGCTCAGTTCTGGATGAATCTCC		 803 PCR, flanking
higBPa Down-PA7 (+)GCATCGCCGATTCCAAGTG
(−)GCAACGTGTGTTCGTCACC		 655 PCR, flanking

(+) sense primer

(−) antisense primer