Skip to main content
. Author manuscript; available in PMC: 2012 Oct 5.
Published in final edited form as: J Am Chem Soc. 2011 Sep 13;133(39):15316–15319. doi: 10.1021/ja2066913

Figure 1.

Figure 1

Selective PEGylation of HPG-Ub with an mPEG-linked phenyl iodide 1n via copper-free Sonogashira cross-coupling. (a) Reaction scheme. (b) Coomassie blue staining of SDS-PAGE gel showing selective formation of the PEGylated Ub (Ub-mPEG): lane 1, control reaction with wild-type ubiquitin; lane 2: control reaction with HPG-Ub in the presence of 50 equiv of 1n but absence of palladium complex; lane 3: reaction with HPG-Ub in the presence of 50 equiv of 1n and palladium catalyst.