Table 2.
Q-rtPCR validation of 11 genes from the CRY list
| Rank in array | Gene name | Lens (L/S) | CLT (R/S) |
|
|---|---|---|---|---|
| qPCR | qPCR | array | ||
| 1 | Cryba1 | 37009.6 | 38.5 | 4.5 |
| 3 | Crygb (sim) | 75864.3 | 7.3 | 8.1 |
| 4 | Crybb3 | 45918.7 | 7.8 | 6.2 |
| 6 | Cryaa | 95666.6 | 17.9 | 1.1 |
| 7 | Glb1 | 7.6 | 0.8 | 1.3 |
| 8 | Cugbp1b | 41.8 | 1.3 | 2.4 |
| 15 | Ccdc124 | 3.62 | 1.5 | 1.1 |
| 18 | Crybb1 | 22333.3 | 5.0 | 9.6 |
| 26 | Wnt7a | 39193.7 | 0.5 | 1.1 |
| 29 | Fzd7* | 5.9 | 3.9 | 1.3/2.7 |
| 36 | Kif19 | 2.1 | 0.5 | 1.2 |
Bold numbers indicate upregulation of more than 1.5 fold, numbers in italics indicate down regulation and normal font indicates no significant change. * appears twice in CRY list. L/S shows the relative (fold higher) expression in lens when compared to sham operated corneas. Likewise R/S shows relative expression in corneas undergoing CLT when compared to sham operated control corneas.