Figure 2. Effect of single treatment with METH, cocaine, MDMA or morphine on the extracellular dopamine level in the VTA/NAc/mPFC triple slice co-cultures.

The triple slice co-cultures were treated with METH (0.1–1000 µM; A), cocaine (0.1–300 µM; B), MDMA (0.1–1000 µM; C) or morphine (0.1–1000 µM; D) for 30 min in KRH buffer, and then the extracellular dopamine levels were determined. Values represent means of the dopamine concentration ± S.E.M. METH, n = 3–7; cocaine, n = 3–10; MDMA, n = 3–5; morphine, n = 3–5. *p<0.05, **p<0.01, ***p<0.001 versus PBS-treated slice co-cultures.