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. 2011 Sep 30;6(9):e24746. doi: 10.1371/journal.pone.0024746

Figure 1. Distribution of small RNAs in three developmental stages of S. frugiperda and annotation of mapped rasiRNAs.

Figure 1

A) Total RNAs (5 µg) from three developmental stages of S. frugiperda were loaded onto a 17% polyacrylamide gel containing 7 M urea and stained with silver after electrophoretic separation. Molecular sizes (in nucleotides) are shown on the left of the figure. Some discrete bands of small RNAs are labeled with asterisks. 1) Decade small RNA ladder, 2) total RNA 2.5 days old fertilized eggs. 3) total RNA L2 larvae, 4) total RNA 12 days old pupae, 5) small synthetic RNA 21 nt, 6) small synthetic RNA 36 nt. B) Pie charts summarizing the annotation of 102 rasiRNAs, mapped on consensus DNA repeated elements (upper panel) and the annotation of consensus DNA repeated elements defined after analysis of 1% of the sequenced S. frugiperda genome (lower panel).