Figure 4. Northern blot analysis of LNCR expression in S. frugiperda adults.
Total RNA (15 micrograms) extracted from S. frugiperda adults was denatured, separated on 1.2% agarose gel (lane 2), together with RNA ladder (lane 1) and stained with EtBr. Molecular sizes of RNA ladder (in nucleotides) are indicated on the left of the figure. The RNA was blotted onto nylon membrane and hybridized with a 32P-labeled LNCR specific DNA probe. The probe (1244 bp) was generated with ncRNA_long primers (see Material and Methods) by PCR, using cDNA of adults as template. Northern blot is shown in lane 3.