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. 2011 Sep 30;6(9):e25284. doi: 10.1371/journal.pone.0025284

Figure 3. Knockdown of CAAP induces caspase-dependent apoptosis in A549 cells.

Figure 3

(A) The expression levels of CAAP in lung cancer A-549 cells were knocked down by the siRNA's, si67 and si48 relative to a non-targeting control siRNA at the indicated concentrations. (the higher the bar the more confident the prediction). (B) Determination of apoptosis in A-549 following transfection with the two CAAP siRNAs si67 and si48 compared to the apoptosis in the non-targeting control siRNA at the indicated concentrations. (C) Determination of the fold increase in caspase-3, -8, -9 and -10 activities following CAAP knockdown by si67 (65 nM) compared to the Ncontrol siRNA (65 nM). (D) Determination of apoptosis in A549 cells 24 hours following CAAP knockdown by si67 in the absence or presence of 75 µM of IETD-FMK (caspase-8 inhibitor), AEVD-FMK (caspase-10 inhibitor), or Z-VAD-FMK, a pan-caspase inhibitor. (E) Determination of apoptosis in A549 cells 24 hours following CAAP knockdown by si67 in the absence or presence of DEVD-FMK (caspase-3 inhibitor).