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. 2011 Sep 30;6(9):e25284. doi: 10.1371/journal.pone.0025284

Figure 5. Apoptosis induced by CAAP knockdown proceeds independently of death receptor pathway.

Figure 5

(A) Western blot analysis for expression of dominant-negative FADD (FADD-DN) in A-549 cells that were transfected with an empty vector or the FADD-DN construct. β-actin expression was determined as a control for loading. (B) Determination of apoptosis of A-549 cells that were transfected with empty vector or FADD-DN. After 24 hrs cells were transfected with si67 or treated with TRAIL (35 ng/ml). The percentage of apoptotic cells was determined after an additional 24 hrs. Apoptosis values for the si67-65 nM transfected cells or the TRAIL treated cells are expressed relative to the amount of apoptosis induced in the vector- or FADD-DN transfected cells. These data demonstrate that FADD-DN overexpression blocks TRAIL induced apoptosis but not apoptosis induced by CAAP knockdown.