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. 2011 Sep 30;6(9):e25284. doi: 10.1371/journal.pone.0025284

Figure 7. Western blot analysis of Cleavage of Bid after knockdown of CAAP in the MCF-7 cell lines and A-549 cells.

Figure 7

(A) The upper panels show Bid cleavage was observed in each of the MCF-7 cell lines after knockdown of CAAP by transfection with si67 at 22 nM, but not in the Ncontrol lanes. In addition to the 13 kDa fragment, observed in each of the MCF-7 lines after knockdown of CAAP, an 11 kDa tBid fragment was detected in the MCF-7/casp3-10b cells. This is consistent with the observation that only in the MCF-7/casp3-10b cell line was significant apoptosis induced by knockdown of CAAP. (B) Although cleavage of Bid was observed in the treatment with both si67 and si48 transfection at both doses, the cleavage bands in the A-549 cells were weaker than in the MCF-7 cell lines.