Figure 4. TGF-β induced DNMTs is associated with growth of prostate cancer in vivo.

A. IVIS 100 imaging system was used to monitor tumor growth in real-time. We found that tumor growth is inhibited dramatically with the treatment of 1D11 compared with Group 2 (13C4 treatment) and 3 (No treatment control). B. 1D11 treatment inhibits the tumor growth in a time dependent manner. C, At the end of the 45-day treatment period, mice were sacrificed and tumors were isolated. The average tumor weight and volume was 5.3 g and 6.85 cm³, respectively in 1D11 treatment group. In comparison, the average weight and volume of tumors in the 10 animals treated with the control 13C4 was significantly greater at 15.8 g and 12.85 cm³, respectively. The corresponding values in the mice that received no treatment were 31.4 g and 23.39 cm³, respectively (P<0.01 among three groups). D. Immunohistochemical analyses of the primary tumors revealed that the expression of p-ERK, DNMTs in animals with 1D11 treatment is significantly lower than those of the other two groups.