Table 1. Bacterial strains, plasmids and cosmids.
Strain or plasmid | Description | Transposon insertiona (genome position), Genbank accession | Reference/Source |
Strains | |||
S. coelicolor A3(2) M145 | Prototrophic SCP-1 SCP-2 Pgl+ | [29] | |
DSCO0600, sigB− | M145 sigB−::Tn5062 (apra) | SC5G5.1.C05 (639940) | [35] |
K101, sigF− | M145 sigF−::apra | [14] | |
sigH− | M145 sigH−::thio | [9] | |
DSC03068, sigI− | M145 sigI−::Tn5062 (apra) | 7F11.01.F06 (3361076) | This study |
DSCO7314, sigM− | M145 sigM−:: Tn5062 (apra) | SC5F8.2.C11 (8120634) | This study |
sigN− (K100) | M145 sigN−::apra | [14] | |
sigK− | M145 sigK−::kan | [36] | |
DSCO0600/DSCO5243, sigB/H | M145 sigB−::apra, sigH::thio | This study | |
J2402, whiB− | M145 whiB−::hyg | [37] | |
J2400, whiG− | M145 whiG−::hyg | [37] | |
JM109 | F′ traD36 proA+B+ lacIq Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14- gyrA96 recA1 relA1endA1 thi hsdR17 | [38] | |
ET12567 (pUZ8002) | dam13::Tn9 dcm6 hsdM hsdR recF143 16zjj201 ::Tn10 galK2 galT22 ara14 lacY1 xyl5 leuB6 thi1 tonA31 rpsL136 hisG4 tsx78 mtli glnV44, containing the non-transmissibleoriT mobilizing plasmid,pUZ8002 | [39] | |
BW25113 (pIJ790 | K12 derivative: deltaaraBAD, deltarhaBAD containing lambdaRED recombination plasmid pIJ790 | [30] | |
Plasmids | |||
pQM5062 | pMOD+Tn5062, AmpicillinR and AramycinR | AJ566337.1 | [32] |
pDpsA6A | dpsA::mCherry, ApramycinR | This study | |
pDpsA6H | dpsA::mCherry, HygromycinR | [15] | |
pDpsA7 | dpsA::His6, ApramycinR | [15] | |
pDpsA7H | dpsA::His6, HygromycinR | [15] |
Access http://strepdb.streptomyces.org.uk/ for information about transposon insertion details.