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. 2011 Sep 30;6(9):e25593. doi: 10.1371/journal.pone.0025593

Table 1. Bacterial strains, plasmids and cosmids.

Strain or plasmid Description Transposon insertiona (genome position), Genbank accession Reference/Source
Strains
S. coelicolor A3(2) M145 Prototrophic SCP-1 SCP-2 Pgl+ [29]
DSCO0600, sigB M145 sigB::Tn5062 (apra) SC5G5.1.C05 (639940) [35]
K101, sigF M145 sigF::apra [14]
sigH M145 sigH::thio [9]
DSC03068, sigI M145 sigI::Tn5062 (apra) 7F11.01.F06 (3361076) This study
DSCO7314, sigM M145 sigM:: Tn5062 (apra) SC5F8.2.C11 (8120634) This study
sigN (K100) M145 sigN::apra [14]
sigK M145 sigK::kan [36]
DSCO0600/DSCO5243, sigB/H M145 sigB::apra, sigH::thio This study
J2402, whiB M145 whiB::hyg [37]
J2400, whiG M145 whiG::hyg [37]
JM109 F′ traD36 proA+B+ lacIq Δ(lacZ)M15/Δ(lac-proAB) glnV44 e14- gyrA96 recA1 relA1endA1 thi hsdR17 [38]
ET12567 (pUZ8002) dam13::Tn9 dcm6 hsdM hsdR recF143 16zjj201 ::Tn10 galK2 galT22 ara14 lacY1 xyl5 leuB6 thi1 tonA31 rpsL136 hisG4 tsx78 mtli glnV44, containing the non-transmissibleoriT mobilizing plasmid,pUZ8002 [39]
BW25113 (pIJ790 K12 derivative: deltaaraBAD, deltarhaBAD containing lambdaRED recombination plasmid pIJ790 [30]
Plasmids
pQM5062 pMOD+Tn5062, AmpicillinR and AramycinR AJ566337.1 [32]
pDpsA6A dpsA::mCherry, ApramycinR This study
pDpsA6H dpsA::mCherry, HygromycinR [15]
pDpsA7 dpsA::His6, ApramycinR [15]
pDpsA7H dpsA::His6, HygromycinR [15]
a

Access http://strepdb.streptomyces.org.uk/ for information about transposon insertion details.