Table 1. List of genes whose expression was investigated by qRT-PCR.
Gene | Function | Primer names | Primer sequences |
UreA | Urease A (HP0073, of ureA-B | HP0073P1 | GTTAGACAAGTTGATGCTCC |
operon) | HP0073P2 | CCATCAGGAAACATCGCTTC | |
UreI | Accessory protein for urease | HP0071P1 | CCACCCTACAGCCCCTG |
(HP0071of ureIEFGH operon) | HP0071P2 | CGCAGCAGGAATCGTGTTG | |
FucST | Fucose synthase (HP0044) | HP0044P5 | (AAGACGGATCC)ATGAAAGAAAA |
AATCGCTTTAATCAC | |||
HP0044P7 | CTGTAGGCTTAGTGGTAGCGA | ||
FucT | α-1,3- fucosyl transferase | HPFucTP1 | CTGGGGGAGGAGCCGTG |
(HP0379) | HPFucTP2 | GGGTGCGTGTGCAAGTATC | |
GalT | β-1,4- galactosyl transferase | HP0826P1 | GCCACCCACCACCAAGCGC |
(HP0826) | HP0826P2 | CACCCAAGCTCCCCCAAG | |
WaaL | O-antigen ligase (HP1039) | HP1039P9 | (GGAATTC)GTGTTGAAAGAGCGTT |
TGAAAGCC | |||
HP1039P4 | (GAAGATCT)AAACATGTTAGGGAA | ||
GATGCT | |||
FlaA | Flagellin A (HP0601) | FlaA5 | ATGGCTTTTCAGGTCAATAC |
FlaA4 | CATCCATAGCCTTATCCGC | ||
FlaB | Flagellin B (HP0115) | HP0115P1 | GGATAAATACCAATATCGCC |
HP0115P2 | CATCCATCGCTTTATCTGC | ||
FlhA | Basal body and transcriptional | HP1041P1 | GGGCCTAGTGCGGTGAG |
regulator (HP1041) | HP1041P2 | CCGCATCAATCGCCATTTG | |
CoAST | AcCoA synthetase (HP1045) | HP1045P1 | GTCATTATCTATATGCCCAT |
HP1045P2 | CTGGCTTGAGCATGTAAGG |
The functions of the genes are indicated, as well as the names and sequences of the primers used. Nucleotides in brackets correspond to polynucleotide tails and restriction sites included for other cloning purposes.