Skip to main content
. 2011 Sep 30;6(9):e25722. doi: 10.1371/journal.pone.0025722

Table 1. List of genes whose expression was investigated by qRT-PCR.

Gene Function Primer names Primer sequences
UreA Urease A (HP0073, of ureA-B HP0073P1 GTTAGACAAGTTGATGCTCC
operon) HP0073P2 CCATCAGGAAACATCGCTTC
UreI Accessory protein for urease HP0071P1 CCACCCTACAGCCCCTG
(HP0071of ureIEFGH operon) HP0071P2 CGCAGCAGGAATCGTGTTG
FucST Fucose synthase (HP0044) HP0044P5 (AAGACGGATCC)ATGAAAGAAAA
AATCGCTTTAATCAC
HP0044P7 CTGTAGGCTTAGTGGTAGCGA
FucT α-1,3- fucosyl transferase HPFucTP1 CTGGGGGAGGAGCCGTG
(HP0379) HPFucTP2 GGGTGCGTGTGCAAGTATC
GalT β-1,4- galactosyl transferase HP0826P1 GCCACCCACCACCAAGCGC
(HP0826) HP0826P2 CACCCAAGCTCCCCCAAG
WaaL O-antigen ligase (HP1039) HP1039P9 (GGAATTC)GTGTTGAAAGAGCGTT
TGAAAGCC
HP1039P4 (GAAGATCT)AAACATGTTAGGGAA
GATGCT
FlaA Flagellin A (HP0601) FlaA5 ATGGCTTTTCAGGTCAATAC
FlaA4 CATCCATAGCCTTATCCGC
FlaB Flagellin B (HP0115) HP0115P1 GGATAAATACCAATATCGCC
HP0115P2 CATCCATCGCTTTATCTGC
FlhA Basal body and transcriptional HP1041P1 GGGCCTAGTGCGGTGAG
regulator (HP1041) HP1041P2 CCGCATCAATCGCCATTTG
CoAST AcCoA synthetase (HP1045) HP1045P1 GTCATTATCTATATGCCCAT
HP1045P2 CTGGCTTGAGCATGTAAGG

The functions of the genes are indicated, as well as the names and sequences of the primers used. Nucleotides in brackets correspond to polynucleotide tails and restriction sites included for other cloning purposes.