Table 2. Identification of candidate glycoproteins by ProQ-emerald staining.
| GP # | Spot #(MWa) | ProQ/Cy5WT | ProQ/Cy5hp0366 | ORF | Function | %b | MWb | N-glyc sequond |
| 1 | 14 | 0.25 | 0.19 | HP1588 | Conserved unknown | 51 | 28.4 | None |
| 2 | (28.5) | HP0879 | Flagellar chaperone FliT | 37 | 28.3 | 2, G | ||
| 3 | 15 | 0.25 | 0.19 | HP1043 | Transcriptional regulator | 64 | 25.5 | 1, G |
| 2 | (28.5) | HP0879 | Flagellar chaperone FliT | 53 | 28.3 | 2, G | ||
| 4 | 33 | nd | nd | HP1132 | F0F1 ATP synthase subunit | 73 | 51.5 | 1, G |
| (52.1) | β | |||||||
| 5 | 61 | 0.24 | 0.25 | HP0026 | Citrate synthase GltA | 44 | 48.3 | 1, G |
| (53.6) | ||||||||
| 5 | 62 | 0.28 | 0.28 | HP0026 | Citrate synthase GltA | 49 | 48.3 | 1, G |
| (53.6) | ||||||||
| 6 | 70 | 0.21 | 0.18 | HP1134 | F0F1 ATP synthase subunit | 32 | 55.1 | 1, G |
| (62.3) | α | |||||||
| 7 | 72 | 0.19 | 0.17 | HP0900 | Hydrogenase HypB | 51 | 27.3 | 1, G |
| (30.6) | ||||||||
| 7 | 73 | 0.20 | 0.20 | HP0900 | Hydrogenase HypB | 40 | 27.3 | 1, G |
| (30.6) | ||||||||
| 8 | 77 | 0.33 | 0.23 | HP1037 | X-Pro dipeptidase | 32 | 40.8 | None |
| (42.6) | ||||||||
| 8 | 80 | 0.19 | 0.22 | HP1037 | X-Pro dipeptidase | 44 | 40.8 | None |
| (42.6) | ||||||||
| 9 | 78 | 0.20 | 0.15 | HP1555 | Elongation factor EF-Ts | 54 | 39.7 | None |
| (44.3) | ||||||||
| 9 | 79 | 0.24 | 0.13 | HP1555 | Elongation factor EF-Ts | 50 | 39.7 | None |
| (44.3) |
Total proteins were labeled with Cy5, resolved by 2D gel electrophoresis and glycoproteins were labeled by ProQ-emerald staining. The ratios of ProQ-emerald to Cy5 staining of the wild-type and hp0366 mutant strains and the protein identities are indicated for the 12 spots identified as GP candidates so far. Because the same protein hits were obtained for different spots, this results in the identification of 9 GP candidates. The spot numbers refer to Figure 3.
average molecular weight calculated from 2 independent ProQ-emerald-stained gels.
% coverage of the protein sequence for MS identification.
molecular weight as per amino acid sequence, in the absence of glycosylation.
N-glyc sequon: each ORF was examined for the presence of a N-glycosylation sequon of the general NxS/T type or extended bacterial type D/ExNxS/T where x is any amino acid except proline. The number of sequons is indicated, as well as their type: G, for general.
nd: ratio not determined due to smear from abundant neighbouring spot upon Cy5 detection.