Table 1. Tellurite exposure results in augmented NADPH levels in E. coli.
Treatment | |||
Cofactor | Control | K2TeO3 (2 µM) | Menadione (100 µM) |
NADPHa | 119.1±1.4 | 151.0±6.6* | 155.2±9.1* |
NADP+ | 46.9±7.8 | 47.7±5.0 | 44.4±6.9 |
NADH | 53.0±3.1 | 27.0±4.9* | 22.3±2.1* |
NAD+ | 48.4±0.4 | 43.9±6.4 | 55.0±2.3 |
NADP(H) (nmol mg prot−1) and NAD(H) (mmol mg prot−1) concentration in E. coli BW25113 extracts was determined spectrophotometrically at 340 nm as described in Methods. Values are the mean of 3 independent trials ± SD.
*P≤0.05 as compared to controls.