Table 2. Tellurite induces NADPH-dependent enzymatic activities in E. coli.
BW25113 | Δzwf | |||
Enzyme | Control | K2TeO3 | Control | K2TeO3 |
ICDH | 0.095±0.01 | 0.121±0.01* | 0.011±0.01 | 0.084±0.04* |
GDH | 7.7±0.7 | 11.8±1.3* | 0.3±0.07 | 0.6±0.1* |
G6PDH | 7.0±1.4 | 11.3±1.7* | ND | ND |
Enzymatic activity (µmol NADPH min−1 mg prot−1) was determined spectrophotometrically at 340 nm as described in Methods. Values are the mean of 4 independent trials ± SD.
*P≤0.05 as compared to controls. ND, not detected.