Table 4. Effect of zwf expression and toxicant exposure on NADPH concentration.
| Strain | Plasmid | Treatment | NADP+a | NADPHa | [NADP++NADPH] |
| BW25113 | pBAD | Control | 46.9±7.8 | 119.1±7.7 | 166.0 |
| K2TeO3 | 47.7±5.0 | 157.0±6.6* | 204.8 | ||
| Menadione | 44.4±6.9 | 155.2±9.1* | 199.6 | ||
| BW25113 | pBAD-zwf | Control | 35.0±3.5 | 156.8±13.5 | 191.8 |
| K2TeO3 | 47.1±1.9* | 218.3±6.6* | 265.4 | ||
| Menadione | 42.6±4.6 | 229.6±10.0* | 272.2 | ||
| Δzwf | pBAD | Control | 68.7±3.2 | 100.4±2.8 | 169.1 |
| K2TeO3 | 70.2±8.2 | 124.4±10.0 | 194.6 | ||
| Menadione | 73.8±6.8 | 98.1±8.7 | 172.4 | ||
| Δzwf | pBAD-zwf | Control | 45.5±4.1 | 138.8±3.0 | 184.3 |
| K2TeO3 | 57.9±3.1 | 156.0±8.5* | 214.1 | ||
| Menadione | 46.8±8.3 | 141.9±4.0 | 188.7 |
a
nmol/mg protein. Values are the mean of 3 independent trials ± SD.
*
, P≤0.05 as compared to control.