Figure 2. Characterization of expression of the α₁-subunits by Western-blot analysis in cytosolic fractions of Sf-9 cells infected with the respective variants of the α₁-subunits.

All lanes were loaded with 40 µg protein of the cytosolic fractions. The experiments were repeated at least three times and one representative result is shown. [molecular weights predicted from their amino acid sequences: α₁WT, 77.6 kDa; α₁ΔN₂₃₆, 51.2 kDa; α₁ΔN₂₅₉, 48 kDa].