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. 2011 Sep 30;6(9):e25772. doi: 10.1371/journal.pone.0025772

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Kraehling et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Mean fluorescence lifetimes of ECFP were measured in HEK-293 cells at 37°C, 48 h post transfection with the respective recombinant constructs. A: α₁-variants as fluorescence-donor. B: α₁-variants as fluorescence-acceptor. Data are presented in box and whiskers plots showing the 25^th percentiles, 75^th percentile and median as box with the mean value as dotted line. Whiskers represent the 5^th and 95^th percentile, dots are outliers. Fluorescent tagged NOsGC variants show significantly reduced fluorescent lifetimes due to FRET.