Figure 9. Expression of EYFP-marked α₁ full-length (A) and C-α₁ (B) in HEK-293 cells.

As a marker of the endoplasmic reticulum [11] ECFP tagged heme oxygenase 1 (human, HO-1) was co-transfected. A, α₁ full-length shows cytosolic distribution. B, C-α₁ shows a similar distribution like ECFP-HO-1. C, addition of an untagged β₁-subunit led in some cells a similar subcellular distribution as the wild type. The red arrow denotes a cell likely co-transfected with β₁, the white arrow denotes a cell likely not co-transfected with β₁. D, only cells which express both subunits show homogenous subcellular distribution. The red arrow denotes a cell co-transfected with β₁, the white arrow denotes a cell not co-transfected with β₁. E, The subcellular localization of α₁ full-length is not affected through coexpression of β₁. Bar, 20 µm. CFP, ECFP channel; YFP, EYFP channel.