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. 2011 Sep 30;6(9):e25653. doi: 10.1371/journal.pone.0025653

Figure 1. Experimental design.

Figure 1

(A) The experimental set-up consist of unilateral injections of 6-OHDA into the right medial forebrain bundle anterior of the substantia nigra. A so-called cylinder test was used to assess forelimb asymmetry use and indicated the degree of DA-denervation. L-DOPA treatment and dyskinesia rating (blue arrow; 8 mg/(kg and day) commenced one day after the first cylinder test and ended one day after the second test. (B) The brains were dissected out 60 minutes after the last L-DOPA dose. Sections cut on a cryostat were thaw mounted onto MALDI compatible glass slides and 80 pL-sized drops of MALDI matrix placed in arrays across the sections. (C) Mass spectra were collected from every matrix spot and assembled into a MALDI imaging MS file that was used to visualize the distribution of different peptides, lipids and small proteins (displayed in yellow, green, and red).