Figure 5.
Effect of REST-inhibition on hepatocytes. (A) Comparable transfection in REST-inhibited (R) and luciferase control (C) groups as assessed by GFP. (B) Photomicrographs of REST-inhibited(R) or control (C) hepatocytes over a time course. Original magnification 5x. (C) Cell survival as assessed by live mitochondrial (MTT) assay between control (C) and REST-inhibited (R) hepatocytes over a time course. (D) % apoptotic nuclei measured 3 days after transfection by TUNEL assay. (E) Rate of proliferation as assessed by tritiated thymidine incorporation 24h after transfections. *Significantly different from R group.