Table 1.
The numbers of proper strains among all single, double, triple, and quadruple deletion mutants generated from the backbone models for E. coli (iBKEco52) and S. cerevisiae (iBKSce50, iBKSce50Δmit, and iBKSce50+7)
| Target higher alcohols | Number of proper strains Total (single/double/triple/quadruple deletion mutants) |
|||
|---|---|---|---|---|
| iBKEco52 | iBKSce50 | iBKSce50Δmit | iBKSce50+7 | |
| 1-propanol from OAA | 458 (0/11/85/362) |
40 (2/16/14/8) |
108 (4/27/50/27) |
467 (4/23/128/312) |
| 1-Butanol from AcCoA | 347 (1/17/87/242) |
1 (1/0/0/0) |
49 (2/7/17/23) |
192 (33/22/44/93) |
| 1-Butanol from OAA | 501 (0/10/89/402) |
71 (0/10/30/31) |
120 (3/25/59/33) |
373 (2/17/116/238) |
| Isobutanol from PYR | 501 (1/14/83/403) |
38 (2/15/21/0) |
85 (4/25/30/26) |
276 (2/18/81/175) |
| 3-Methyl-1-butanol from PYR | 208 (2/13/51/142) |
46 (1/15/21/9) |
98 (4/25/46/23) |
94 (2/5/33/54) |
| Isopentenol from AcCoA | 330 (0/5/55/270) |
0 (0/0/0/0) |
104 (0/12/51/41) |
166 (2/10/54/100) |
The "proper" strains were defined as viable target-producing strains in which all deletions contributed to the improvement of product yields. The growth rates and product yields were determined by FBA-based metabolic simulations. iBKSce50Δmit is an hypothetical S. cerevisiae model derived by merging the cytosolic and mitochondrial networks into one compartment. iBKSce50+7 is a model of iBKSce50 expanded by the addition of 7 E. coli reactions.