FIG. 3.

Effect of ACK2 and VCD on ovarian pKIT is shown. Ovaries from PND4 rats were precultured with control medium with or without ACK2 (80 μg/ml) for 4 h and then VCD (30 μM) was added, or not, to the culture for 2 days. A) Representative Western blots for pKIT, KIT, and ACTB expression in cultured ovaries in control medium (C1, C2, C3) or medium containing ACK2 (A1, A2, A3), VCD (V1, V2, V3), or VCD plus ACK2 (VA1, VA2, VA3) are shown. B) pKIT (left panel) and total KIT (right panel) protein intensity values were normalized to those of ACTB protein and expressed as means ± SEM; n = 3; 15 ovaries/pool for cultured ovary samples; different superscript letters indicate differences (P < 0.05) between the groups.