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Measurement of progesterone (P4) production in the granulosa cell conditioned media after 24 h of culture in the presence or absence of the MMP2/9 inhibitor. Human chorionic gonadotropin-induced P4 production (mean ± SEM) resulting in ∼70-fold increase; 0 h (control) = 0.7 ± 0.11 ng/ml, 24 h hCG (+hCG) = 48.0 ± 1.59 ng/ml. Treatment with the MMP2/9 inhibitor (hCG+Inh) had no effect on hCG-stimulated P4 production.
