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. Author manuscript; available in PMC: 2012 Oct 7.
Published in final edited form as: Org Lett. 2011 Sep 9;13(19):5140–5143. doi: 10.1021/ol202020c

Figure 3. Luciferase Based Assay for SHH Activity.

Figure 3

SHH-Light2 cells were treated as described.18 GLI-binding site luciferase activities were measured using the luciferase reporter assay system with the luciferase kit (Promega). Treatment of SHH-Light2 cells with recombinant SHH (200 ng, R&D) resulted in the strong induction of reporter activity, which was largely blocked by co-treatment with either cyclopamine 1 or with 11 at 5 μM (in DMSO), both P < 0.001 [SHH vs. SHH + 1; SHH vs. SHH + 11].