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. Author manuscript; available in PMC: 2012 Mar 11.
Published in final edited form as: Nat Methods. 2011 Sep 11;8(10):837–839. doi: 10.1038/nmeth.1700

Figure 2. Feedback control can decrease cell-to-cell variability in optogenetic response.

Figure 2

(a) The schematic depicts that the same light input applied to cells expressing different levels of optogenetic components will lead to different activity levels. Cell-by-cell light adjustment is necessary to achieve uniform membrane-bound PIF concentrations. (b) Histograms of PIF membrane recruitment under a constant light input (0.2 V 650 nm, 0.1 V 750 nm; blue curve) and during feedback control (green curve). (c) The feedback-controlled voltages applied over time to each cell in b. The light inputs that are required to compensate for cell-cell heterogeneity span a large range of intensities. (d-e) The mean (d) and standard deviation (e) of PIF recruitment in the presence or absence of feedback control for the cell populations shown in b. AU: arbitrary units.