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. 2001 Mar 27;98(8):4385–4390. doi: 10.1073/pnas.071411598

Figure 6.

Figure 6

Overexpression of nm23H1 reduces the adhesion-stimulated membrane ruffles of Rat1 cells. (a–d) Rat1 cells were stained for Tiam1 (a and c) and for F-actin (b and d) on uncoated slides (a and b) or replated on fibronectin-coated slides for 15 min (c and d). (e–j and l–o) Rat1 cells stably expressing GFP (e, g, i, l, and n) or GFP-tagged nm23H1 (f, h, j, m, and o) were either untransfected (e–j) or were transfected with Tiam1 C1199 (myc tagged at the C terminus) (l–o), then replated on fibronectin-coated slides for 15 min and stained for F-actin (e, f, l, and m), endogenous Tiam1 (g and h), or Tiam1 C1199 (anti-myc) (n and o). The same cells are shown in a–d, g, i, and h, j. (i and j) Stable expression of GFP or GFP-nm23H1. Expression of endogenous or transfected Tiam1 and nm23H1 in Rat1 cells is shown by immunoblot (k and p). Three independent clones expressing GFP-tagged nm23H1 or GFP were examined, and similar results were obtained.