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Direct interactions between HDA1, HDA2, and HDA3. 35S-Methionine-labeled HDA1, HDA2, and HDA3 were incubated with GST (lanes 2, 5, and 8) or GST-HDA1 (lanes 3, 6, and 9) and immobilized on glutathione-Sepharose beads. 35S-Methionine-labeled HDA2 was incubated with GST (lane 11) and GST-HDA3 (lane 12). The interactions were analyzed according to Materials and Methods.
