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HDA2 and HDA3 are essential for repression by HDA1. Expression of ENA1-LacZ was derepressed in hda1Δ, hda2Δ, and hda3Δ mutants. Plasmid pFR70i harboring ENA-LacZ was integrated into the URA3 loci of the chromosomes of WT (YDS2), hda1Δ (WJY110), hda2Δ (WJY103), and hda3Δ (WJY104). β-Galactosidase activity is shown in Miller units. Values represent averages for 4–6 transformants. Standard errors were <5%.
