Table 1.
Purification step | Total protein (mg) | Total activity (IU) | Specific activity (IU·mg−1) | Purification (fold) | Yield (100%) |
---|---|---|---|---|---|
Crude enzyme | 11668.43 | 127.96 | 0.011 | 1 | 100 |
Acetone precipitation | 509.23 | 80.43 | 0.16 | 14.5 | 62.8 |
Sephadex G-100 | 120.43 | 30.64 | 0.25 | 22.7 | 23.9 |